The wreathed hornbill (Rhyticeros undulatus) is a bird species from the Bucerotidae family that is protected due to its declining population. Ex-situ conservation efforts are being carried out to help recover this species; however, challenges such as the rise of antimicrobial resistance complicate these efforts. This study aims to identify Enterobacteriaceae bacteria from the feces of wreathed hornbills in ex-situ conservation areas and to test their susceptibility to antimicrobials.